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Status
Public on Oct 23, 2004
Title
CGH4_73, 5cl20, XY
Sample type
genomic
Channel 1 Source name
2ng of Phi-29amplified male sample 5cl20 DNA
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
commercial male DNA (Promega)
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
2ng of sample 5cl20 DNA (XY), containing a know trisomy over 5 clones on chr20, were amplified with of Phi-29 enzyme (GenomiPhi kit-Amersham) in order to simulate the amounts of a microdissected sample. Details of the experiment are available within the Nucleic Acid Research, 2004 manuscript "Genomic profiling by DNA amplification of laser capture microdissected tissues and array CGH", by Cardoso J. et al.
Submission date
Oct 14, 2004
Last update date
May 27, 2005
Contact name
Joana Cardoso
E-mail(s)
joanacardoso@fm.ul.pt Phone
351-21-7999519
Organization name
Faculty of Medicine
Lab
Biology of Chromatin Unit
Street address
Av. Prof Egaz Moniz
City
Lisbon
ZIP/Postal code
1700
Country
Portugal
Platform ID
GPL1506 Series (1)
GSE1841 BAC CGH array profiling using Phi29-amplified microdissected samples
Data table header descriptions ID_REF
VALUE Log2Lowess Norm ratios, array CGH4_73, sample Phi29-amplified 5cl20 DNA (XY) vs. non-amplified male (XY)
Data table ID_REF VALUE 10
0.040103473
11
-0.308239236
20
0.050614195
21
-0.23479261
40
0.061209076
50
-0.276217562
60
0.121359948
61
-0.298389575
62
-0.332037238
70
-0.003890238
71
0.167378303
72
0.235160082
80
0.038701641
90
0.597430438
91
0.494408872
92
0.611714429
100
-0.011602258
101
0.097986012
102
-0.022005104
110
0.509982306
3615 63 Kbytes .Supplementary data files not provided
