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Sample GSM3227629 Query DataSets for GSM3227629
Status Public on Jun 18, 2019
Title MM.154: MM at diagnosis, sample 22
Sample type RNA
 
Source name Highly purified PCs from MM patient
Organism Homo sapiens
Characteristics tissue: Bone marrow plasma cells
diagnosis: Multiple myeloma (MM)
genotype/variation: MAF translocation
Treatment protocol Plasma cells were purified from bone marrow samples using CD138 immunomagnetic microbeads according to the manufacturer's instructions (MidiMACS system, Miltenyi Biotec); the purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL).
Label biotin
Label protocol Biotinylated single stranded cDNA samples were prepared starting from 100-250 nanograms of total RNA, according to the standard Affymetrix protocol (GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual).
 
Hybridization protocol The fragmented labeled single-stranded DNA target was hybridized for 16 hours and 30 minutes at 45°C on GeneChip Gene 2.0 ST array, according to the standard Affymetrix protocol, using the GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Scanning of the arrays was performed using the GeneChip Scanner 7G.
Data processing Normalized expression values were obtained using Robust Multi Array Average (RMA) procedure. A custom annotation pipeline was applied that combined GENCODE v25 (Ensembl v87) annotations with the CDF (Chip Definition File) version 21 for Human GeneChip Gene 2.0 ST transcript annotations freely available at http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/ genecodet.asp, in order to withdraw probes that map to regions where ambiguous detection due to transcript overlap might occur. Only genes identified by probesets with 4 or more specifc probes within were retained. The gained CDF results a umich GENCODEG-like annotation that retains only unambiguous signals for lncRNA and coding genes that partially overlap in thier genomic position.
 
Submission date Jun 26, 2018
Last update date Jun 18, 2019
Contact name Luca Agnelli
E-mail(s) luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
Phone +390223903581
Organization name IRCCS Istituto Nazionale dei Tumori
Department Department of Advanced Diagnostics
Street address Venezian 1
City MILAN
ZIP/Postal code 20133
Country Italy
 
Platform ID GPL25249
Series (1)
GSE116294 Deregulated high expression of NEAT1 lncRNA in multiple myeloma is unrelated to molecular features and clinical outcome
Relations
Alternative to GSM2932345

Data table header descriptions
ID_REF
VALUE RMA-normalized log2 signal

Data table
ID_REF VALUE
ENSG00000000003.14 4.186519955
ENSG00000000005.5 3.839335888
ENSG00000000419.12 11.52439793
ENSG00000000457.13 8.373281864
ENSG00000000460.16 5.506501742
ENSG00000000938.12 5.463876498
ENSG00000000971.15 5.809248181
ENSG00000001036.13 8.737581874
ENSG00000001084.10 8.238217888
ENSG00000001167.14 8.984017646
ENSG00000001460.17 5.233758533
ENSG00000001461.16 6.021961883
ENSG00000001497.16 8.440679137
ENSG00000001561.6 8.852163404
ENSG00000001617.11 6.844196843
ENSG00000001626.14 3.172699466
ENSG00000001629.9 10.17528941
ENSG00000001630.15 6.989727161
ENSG00000001631.15 9.235020889
ENSG00000002016.17 6.481285504

Total number of rows: 35458

Table truncated, full table size 1047 Kbytes.




Supplementary file Size Download File type/resource
GSM3227629_MM.154.CEL.gz 8.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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