2ng of a mixture 1:1 of commercial female DNA (XX) and commercial male DNA (XY) were amplified with Phi-29 enzyme (GenomiPhi kit-Amersham) in order to simulate the amounts of a microdissected sample. Details of the experiment are available within the Nucleic Acid Research, 2004 manuscript "Genomic profiling by DNA amplification of laser capture microdissected tissues and array CGH", by Cardoso J. et al.