|
Status |
Public on Sep 25, 2008 |
Title |
JPA284 |
Sample type |
RNA |
|
|
Source name |
JPA284
|
Organism |
Homo sapiens |
Characteristics |
Juvenile pilocytic astrocytoma Age: 9 Sex: F Location: posterior fossa Histopathology: hypocellular, Ki-67(1-4%)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol followed by RNeasy was performed according to the manufacturer's instructions
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
Scan protocol |
GeneChips were scanned using GeneArray Scanner Model G2500
|
Description |
Gene expression from juvenile pilocytic astrocytoma
|
Data processing |
The data were analyzed using GCOS1.4
|
|
|
Submission date |
Sep 23, 2008 |
Last update date |
Sep 26, 2008 |
Contact name |
Kwong-Kwok Wong |
E-mail(s) |
kkwong@mdanderson.org
|
Phone |
713-792-0229
|
Organization name |
UT M. D. Anderson Cancer Center
|
Department |
Gynecologic Oncology and Reproductive Medicine
|
Street address |
T4-3900, 1515 Holcombe Boulevard
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE12907 |
Expression Analysis of Juvenile Pilocytic Astrocytomas by Oligonucleotide Microarray Reveals Two Potential Subgroups |
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