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Sample GSM3244398 Query DataSets for GSM3244398
Status Public on Jun 01, 2020
Title Sample_17_Wild Type + Chow 1
Sample type SRA
Source name Wild Type + Chow
Organism Mus musculus
Characteristics strain background: C57BL/6J
genotype/variation: wild type
age: 16 weeks
fed state: 10 Hour Fast
tissue/cell type: Hepatocytes
Growth protocol Organisms were housed in flow cages, following the standard 12 hour light/dark cycle
Extracted molecule total RNA
Extraction protocol Whole livers or hepatocytes were isolated, flash frozen on Liquid nitrogen, and RNA was harvested using RNeasy tissue mini-kit (Qiagen, Cat No./ID: 74104)
RNA libraries were prepared for sequencing using standard Illumina protocols for “TruSeq Stranded mRNAseq Sample Prep kit”
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
Description C57_NC_Rep_I_CGTACGTA
Data processing Fastq files were generated and demultiplexed with the bcl2fastq v2.17.1.14 Conversion Software (Illumina)
Adaptors were trimmed from the reads using trimmomatic
Reads were mapped to the genome using STAR_2.4.2a
Insert distribution calculated with Picard_1.119
Gene read_count values were calculated using htseq-counts along with the GRCm38 vM9 annotation file (.gff3).
Genome_build: GRCm38 vM9 mm10 (mus musculus)
Supplementary_files_format_and_content: consists of read_count values calculated for all genes annotated in vM10 for all samples submitted
Submission date Jul 04, 2018
Last update date Jun 01, 2020
Contact name Waqar Arif
Organization name UIUC
Department Biochemistry
Lab 461 Medical School Building
Street address 506 S. Mathews Avenue
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platform ID GPL21103
Series (1)
GSE116627 High-throughput sequencing reveals induction of drug metabolism in various cholestatic livers
BioSample SAMN09601970
SRA SRX4341303

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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