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Sample GSM325444 Query DataSets for GSM325444
Status Public on Feb 01, 2010
Title TS 6.5 day 1
Sample type RNA
 
Source name TS cells differentiated day 1
Organism Mus musculus
Characteristics 129S6 genetic background
Treatment protocol To differentiate TS cell FGF4 heparin and feeder conditioned medium were all withdrawn from the media.
Growth protocol TS cells were grown feeder free with 30% feeder conditioned medium, RPMI, FGF4 and heparin as per Tanaka et al , 1998 PMID:9851926
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A second clean up step was done using the RNEasy kit from QIAGEN according to manufactures instructions
Label biotin
Label protocol as per facility protocols
 
Hybridization protocol as per facility protocols
Scan protocol Gene Chips were scanned using the Hewlett-Packard Gene Array Scanner G2500A.
Description gene expression data of TS cells differentiated day 1
Data processing The data were analyzed with Expression Console (Affymetrix) using the quartile normalization and PLIER algorithm
 
Submission date Sep 30, 2008
Last update date Nov 23, 2009
Contact name Brian Joseph Cox
E-mail(s) b.cox@utoronto.ca
Organization name University of Toronto
Department Physiology
Lab Cox System Biology
Street address 1 King's College Circle, Rm 3360
City Toronto
State/province Ontario
ZIP/Postal code M5S1A8
Country Canada
 
Platform ID GPL1261
Series (2)
GSE12985 Differentiation time course of trophoblast stem cells
GSE12999 Gata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 593.596
AFFX-BioB-M_at 698.691
AFFX-BioB-3_at 570.256
AFFX-BioC-5_at 1076.33
AFFX-BioC-3_at 1131.48
AFFX-BioDn-5_at 2090.34
AFFX-BioDn-3_at 4194.97
AFFX-CreX-5_at 13075.8
AFFX-CreX-3_at 12645.9
AFFX-DapX-5_at 35.7128
AFFX-DapX-M_at 46.0817
AFFX-DapX-3_at 15.2945
AFFX-LysX-5_at 17.6122
AFFX-LysX-M_at 49.5933
AFFX-LysX-3_at 28.3461
AFFX-PheX-5_at 33.1729
AFFX-PheX-M_at 149.065
AFFX-PheX-3_at 120.672
AFFX-ThrX-5_at 38.0555
AFFX-ThrX-M_at 148.551

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM325444.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM325444.chp.gz 323.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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