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Sample GSM325447 Query DataSets for GSM325447
Status Public on Feb 01, 2010
Title TS 6.5 day 4
Sample type RNA
Source name TS cells differentiated day 4
Organism Mus musculus
Characteristics 129S6 genetic background
Treatment protocol To differentiate TS cell FGF4 heparin and feeder conditioned medium were all withdrawn from the media.
Growth protocol TS cells were grown feeder free with 30% feeder conditioned medium, RPMI, FGF4 and heparin as per Tanaka et al , 1998 PMID:9851926
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A second clean up step was done using the RNEasy kit from QIAGEN according to manufactures instructions
Label biotin
Label protocol as per facility protocols
Hybridization protocol as per facility protocols
Scan protocol Gene Chips were scanned using the Hewlett-Packard Gene Array Scanner G2500A.
Description gene expression data of TS cells differentiated day 4
Data processing The data were analyzed with Expression Console (Affymetrix) using the quartile normalization and PLIER algorithm
Submission date Sep 30, 2008
Last update date Nov 23, 2009
Contact name Brian Joseph Cox
Organization name University of Toronto
Department Physiology
Lab Cox System Biology
Street address 1 King's College Circle, Rm 3360
City Toronto
State/province Ontario
ZIP/Postal code M5S1A8
Country Canada
Platform ID GPL1261
Series (2)
GSE12985 Differentiation time course of trophoblast stem cells
GSE12999 Gata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development

Data table header descriptions
VALUE Quantification

Data table
AFFX-BioB-5_at 609.271
AFFX-BioB-M_at 699.679
AFFX-BioB-3_at 566.217
AFFX-BioC-5_at 1107.41
AFFX-BioC-3_at 1183.66
AFFX-BioDn-5_at 2101.63
AFFX-BioDn-3_at 4436.13
AFFX-CreX-5_at 13418.6
AFFX-CreX-3_at 13599.8
AFFX-DapX-5_at 29.4332
AFFX-DapX-M_at 52.0894
AFFX-DapX-3_at 14.7087
AFFX-LysX-5_at 12.3857
AFFX-LysX-M_at 46.8252
AFFX-LysX-3_at 22.6315
AFFX-PheX-5_at 36.2854
AFFX-PheX-M_at 138.988
AFFX-PheX-3_at 103.409
AFFX-ThrX-5_at 51.3356
AFFX-ThrX-M_at 171.144

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.

Supplementary file Size Download File type/resource
GSM325447.CEL.gz 3.9 Mb (ftp)(http) CEL
GSM325447.chp.gz 323.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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