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Sample GSM3308123 Query DataSets for GSM3308123
Status Public on Mar 04, 2019
Title pabociclib resistant cell RNA dataset 1 repeat 1
Sample type RNA
 
Source name T47D cells
Organism Homo sapiens
Characteristics cell type: Immortalized cell line
Treatment protocol Palbociclib resistant cells were grown with 100 nM palbociclib containing medium. Ex-resistant cells were T47D cells, cutured to resistance then grown in drug free medium for 7 weeks +.
Growth protocol Parental T47D cells are were grown as directed by ATCC, resistant cells were cultured in the same way with the addition of 100 nM palbociclib to the medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted using invitrogens recoverall kit according to the manufacturer guidelines
Label biotin
Label protocol HG_U133_2_plus array was carried out as per manufacturers instructions
 
Hybridization protocol HG_U133_2_plus array was carried out as per manufacturers instructions
Scan protocol HG_U133_2_plus array was carried out as per manufacturers instructions
Description Gene Expression data
Data processing Data was normalised using RMA.
 
Submission date Jul 26, 2018
Last update date Mar 04, 2019
Contact name Liam Cornell
E-mail(s) liam_cornell@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Street address 450 brookline avenue
City boston
State/province MASSACHUSETTS
ZIP/Postal code 02215
Country USA
 
Platform ID GPL571
Series (2)
GSE117742 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance (array I)
GSE117748 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance
Relations
Reanalyzed by GSM3308129

Data table header descriptions
ID_REF
VALUE GC-RMA

Data table
ID_REF VALUE
1007_s_at 2306.432552
1053_at 880.030934
117_at 32.179501
121_at 246.828182
1255_g_at 10.533328
1294_at 41.785794
1316_at 186.639311
1320_at 37.094263
1405_i_at 26.27634
1431_at 15.842671
1438_at 105.742151
1487_at 331.852903
1494_f_at 54.175303
1598_g_at 337.080228
160020_at 103.560505
1729_at 307.249142
1773_at 56.029377
177_at 61.624567
179_at 265.373059
1861_at 204.052406

Total number of rows: 22215

Table truncated, full table size 464 Kbytes.




Supplementary file Size Download File type/resource
GSM3308123_2_-_TR1.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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