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Sample GSM3308131 Query DataSets for GSM3308131
Status Public on Mar 04, 2019
Title pabociclib resistant cell RNA dataset 2 repeat 3
Sample type RNA
 
Source name T47D cells
Organism Homo sapiens
Characteristics cell type: Immortalized cell line
Treatment protocol Palbociclib resistant cells were grown with 100 nM palbociclib containing medium. Ex-resistant cells were T47D cells, cutured to resistance then grown in drug free medium for 7 weeks +.
Growth protocol Parental T47D cells are were grown as directed by ATCC, resistant cells were cultured in the same way with the addition of 100 nM palbociclib to the medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted using invitrogens recoverall kit according to the manufacturer guidelines
Label biotin
Label protocol HG_U133_2_plus array was carried out as per manufacturers instructions
 
Hybridization protocol HG_U133_2_plus array was carried out as per manufacturers instructions
Scan protocol HG_U133_2_plus array was carried out as per manufacturers instructions
Description Gene Expression data
Data processing Data was normalised using RMA.
 
Submission date Jul 26, 2018
Last update date Mar 04, 2019
Contact name Liam Cornell
E-mail(s) liam_cornell@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Street address 450 brookline avenue
City boston
State/province MASSACHUSETTS
ZIP/Postal code 02215
Country USA
 
Platform ID GPL571
Series (2)
GSE117743 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance (array II)
GSE117748 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance
Relations
Reanalysis of GSM3308125

Data table header descriptions
ID_REF
VALUE Log-2 GC-RMA

Data table
ID_REF VALUE
1007_s_at 10.77
1053_at 9.36
117_at 4.93
121_at 7.69
1255_g_at 3.35
1294_at 5.11
1316_at 7.18
1320_at 4.97
1405_i_at 3.88
1431_at 3.71
1438_at 6.48
1487_at 8.55
1494_f_at 5.35
1598_g_at 8.18
160020_at 6.12
1729_at 8.18
1773_at 5.63
177_at 5.85
179_at 7.9
1861_at 7.48

Total number of rows: 22215

Table truncated, full table size 345 Kbytes.




Supplementary file Size Download File type/resource
GSM3308131_2_-_TR3.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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