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Sample GSM3308152 Query DataSets for GSM3308152
Status Public on Mar 04, 2019
Title T47D mir-432-5p captured RNA 2
Sample type SRA
 
Source name T47D cells
Organism Homo sapiens
Characteristics cell type: Immortalized cell line
Treatment protocol Biotin labelled miRNA was transfected using lipofectamine 3000
Growth protocol Cells were exponentially gowing on standard, ATCC recommented medium
Extracted molecule total RNA
Extraction protocol RNA was extraced using TRIZOL + column purification
Illumina TruSeq stranded mRNAseq, as per manufacturers guidelines
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description RNA captured using a biotin labelled miR-432-5p, performed in parental T47D cells.
Data processing FKPM was calculated, data presented in the manuscript is dirived from FKPM
FKPM averages for sample repeats which were >0 but <1 was rounded to 1.
DESeq2 analysis was also performed and attached
Genome_build: hg19
Supplementary_files_format_and_content: Excel spreadsheets of both FKPM and DESeq2 are attached
 
Submission date Jul 26, 2018
Last update date Mar 04, 2019
Contact name Liam Cornell
E-mail(s) liam_cornell@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Street address 450 brookline avenue
City boston
State/province MASSACHUSETTS
ZIP/Postal code 02215
Country USA
 
Platform ID GPL18573
Series (2)
GSE117746 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance (RNA-Seq)
GSE117748 MicroRNA-mediated suppression of the TGF-β pathway confers transmissible and reversible CDK4/6 inhibitor resistance
Relations
BioSample SAMN09725021
SRA SRX4474099

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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