|
| Status |
Public on Jan 25, 2019 |
| Title |
FGF19 mouse#2, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Livers from db/db mouse treated with an adeno-associated virus encoding FGF19
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype: db/db
age: 14-week old
treatment: AAV-FGF19
|
| Treatment protocol |
Embryos were dechorionated with 50% bleach, put on a cover slip and covered with Halocarbon oil 27 (Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
db/db mice received a single 200 microliter intravenous injection of 1E11 vector genome adeno-associated virus (AAV) encoding FGF19 or green fluorescent protein via tail vein. Animals were euthanized and livers were collected 2 weeks after injection of the AAV vectors for gene expression analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse livers were snap-frozen in liquid nitrogen upon euthanization of animals. Total RNA was extracted using RNeasy Mini kit (Qiagen) and treated with DNase I according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip WT cDNA synthesis and amplification kit and GeneChip WT terminal labeling kit from Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix mouse gene 1.0 ST whole-transcript Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mouse livers
|
| Data processing |
Data were normalized using robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Jul 30, 2018 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Lei Ling |
| Organization name |
NGM Biopharmaceuticals
|
| Street address |
333 Oyster Point Blvd
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE117855 |
Engineered FGF19 promotes HDL biogenesis and transhepatic cholesterol efflux to prevent atherosclerosis |
|
