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| Status |
Public on Aug 08, 2021 |
| Title |
HCC827 GSK-J4 rep1 |
| Sample type |
SRA |
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| Source name |
HCC827
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| Organism |
Homo sapiens |
| Characteristics |
cell type: Lung adenocarcinoma
agent: GSK-J4
cell line: HCC827
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| Treatment protocol |
DMSO or 10 µM of GSK-J4 is treated for 24h.
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| Growth protocol |
HCC827, H23 and A549 were cultured in DMEM (Welgene, Republic of Korea) supplemented with 10% fetal bovine serum (Welgene, Republic of Korea).
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using RNeasy mini kits (Qiagen, Valencia, CA).
The Libraries were prepared using the TruSeq stranded mRNA sample preparation kit (Illumina).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
HCC827 GSK-J4 rep1
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| Data processing |
Raw reads were mapped to the reference human (hg19) genome assembly with Tophat, and rpkm values were calculated with Cufflink
Genome_build: Hg19
Supplementary_files_format_and_content: RPKM
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| Submission date |
Aug 08, 2018 |
| Last update date |
Aug 08, 2021 |
| Contact name |
Mi Young Kim |
| E-mail(s) |
miyoungkim@kaist.ac.kr
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| Organization name |
KAIST
|
| Street address |
Biological science
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| City |
DaeJeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
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| Platform ID |
GPL18573 |
| Series (2) |
| GSE118319 |
Genome-wide RNA-seq from GSK-J4-treated lung adenocarcinoma cell lines |
| GSE133970 |
Genome-wide assessment of GSK-J4-treated lung adenocarcinoma cell lines |
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| Relations |
| BioSample |
SAMN09784713 |
| SRA |
SRX4521969 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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