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Sample GSM3346368 Query DataSets for GSM3346368
Status Public on Aug 07, 2019
Title NKTR-2-T12
Sample type SRA
 
Source name B16F10 tumors_ACT+NKTR
Organism Mus musculus
Characteristics host strain: C57/BL6
implanted with: B16F10 syngeneic murine melanoma cell line
tissue: B16F10 tumors
treatment: adoptive cell transfer (ACT) of pmel-1 T cells with NKTR-214
time point: day 12 after treatment
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit from Qiagen
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description N2T12_S23
Data processing Reads were mapped using HISAT2 version 2.0.4 and aligned to the Mus musculus genome NCBI build GRCm38.
Reads were quantified HTSeq version 0.6.12 and normalized to fragments per kilobase of exon per million fragments mapped (FPKM) expression values.
Genome_build: Mus musculus genome NCBI build GRCm38
Supplementary_files_format_and_content: Matrix of gene expression measurements in FPKM
 
Submission date Aug 19, 2018
Last update date Aug 07, 2019
Contact name Jennifer Tsoi
E-mail(s) JTsoi@mednet.ucla.edu
Organization name UCLA
Department University of California, Los Angeles
Lab Thomas Graeber
Street address 570 Westwood Blvd, CNSI 4338
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL21493
Series (1)
GSE118748 Improved expansion, functionality and tumor targeting of adoptively transferred T cells with kinetically engineered IL-2
Relations
BioSample SAMN09862038
SRA SRX4575824

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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