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Sample GSM335220 Query DataSets for GSM335220
Status Public on Jun 01, 2009
Title EL4_DMSO_PMAIoM_rep2
Sample type RNA
Source name 4h PMA/IoM Stimulated, DMSO treated EL4 cells
Organism Mus musculus
Characteristics Treatment: DMSO
Treatment: PMA and Ionomycin
cell line: EL4
Treatment protocol EL4 cells were pre-treated with either 10microg/mL cycloheximide (CHX) in DMSO or DMSO for 30 min and stimulated with PMA (10 ng/ml phorbol myristate acetate) and Ionomcyin (1 microM) for 0h or 4h
Growth protocol EL-4 were cultured in RPMI 1640 medium with 10 mM HEPES, 10% fetal calf serum (FCS; CSL, Australia), 120 microg/ml penicillin, and 16 microg/ml gentamycin.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI-reagent as per manufacturers instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
Hybridization protocol As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
Scan protocol Arrays were scanned on an Affymetrix Scanner 3000 7G
Description 4h Stimulated, DMSO treated EL4 cells
Data processing Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
Submission date Oct 20, 2008
Last update date Oct 21, 2008
Contact name Kristine Hardy
Organization name University of Canberra
Lab Cytokine Gene Expression
Street address University of Canberra
City Bruce
State/province ACT
ZIP/Postal code 0200
Country Australia
Platform ID GPL6246
Series (2)
GSE13278 Expression analysis to identify inducible genes in T cells
GSE13279 Defining the chromatin signature of inducible genes in T cells

Data table header descriptions

Data table
10338068 6.228766441
10338069 4.602358818
10338070 4.415307045
10338071 3.935815573
10338072 4.265643597
10338073 5.192661285
10338074 9.821650505
10338075 6.696192265
10338076 7.315364838
10338077 10.89241219
10338078 5.248247147
10338079 6.045007706
10338080 5.147641659
10338081 5.902859688
10338082 5.208704948
10338083 4.283491611
10338084 7.00185585
10338085 9.06853199
10338086 4.750619411
10338087 5.036931992

Total number of rows: 35399

Table truncated, full table size 722 Kbytes.

Supplementary file Size Download File type/resource
GSM335220.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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