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Sample GSM335225 Query DataSets for GSM335225
Status Public on Jun 01, 2009
Title EL4_CHX_unstim_rep3
Sample type RNA
Source name Unstimulated, CHX treated EL4 cells
Organism Mus musculus
Characteristics Treatment: Cycloheximide
Treatment: Unstimulated
cell line: EL4
Treatment protocol EL4 cells were pre-treated with either 10microg/mL cycloheximide (CHX) in DMSO or DMSO for 30 min and stimulated with PMA (10 ng/ml phorbol myristate acetate) and Ionomcyin (1 microM) for 0h or 4h
Growth protocol EL-4 were cultured in RPMI 1640 medium with 10 mM HEPES, 10% fetal calf serum (FCS; CSL, Australia), 120 microg/ml penicillin, and 16 microg/ml gentamycin.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI-reagent as per manufacturers instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol for the Mouse 1.0ST arrays from 100ng total RNA.
Hybridization protocol As per standard Affymetrix protocol for the Mouse 1.0ST arrays.
Scan protocol Arrays were scanned on an Affymetrix Scanner 3000 7G
Description Unstimulated, CHX treated EL4 cells
Data processing Quantile normalisation and Robust Multichip Average (RMA) background correction adjusting for probe sequence (Partek Software) was used to generate gene expression levels from the Mouse Gene 1.0ST arrays.
Submission date Oct 20, 2008
Last update date Oct 21, 2008
Contact name Kristine Hardy
Organization name University of Canberra
Lab Cytokine Gene Expression
Street address University of Canberra
City Bruce
State/province ACT
ZIP/Postal code 0200
Country Australia
Platform ID GPL6246
Series (2)
GSE13278 Expression analysis to identify inducible genes in T cells
GSE13279 Defining the chromatin signature of inducible genes in T cells

Data table header descriptions

Data table
10338068 6.60457325
10338069 4.419495583
10338070 4.544128895
10338071 3.91893959
10338072 3.8814888
10338073 5.379167557
10338074 10.07841396
10338075 6.686315536
10338076 7.405620098
10338077 10.55712795
10338078 5.420834541
10338079 5.095954418
10338080 5.027591228
10338081 6.295036316
10338082 5.588243961
10338083 4.049431324
10338084 7.562021732
10338085 9.318393707
10338086 4.667219162
10338087 5.287391663

Total number of rows: 35399

Table truncated, full table size 722 Kbytes.

Supplementary file Size Download File type/resource
GSM335225.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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