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Sample GSM335280 Query DataSets for GSM335280
Status Public on Oct 16, 2009
Title 6 h HNE+LPS, biological rep2
Sample type RNA
Source name 6 h HNE+LPS
Organism Mus musculus
Characteristics RAW 264.7 cell line
Treatment protocol Samples were treated with 35 μM 4-hydroxy-2-nonenal (HNE). Immediately following treatment, lipopolysaccharide (LPS) was added at a final concentration of 1 μg/mL
Growth protocol Cells were cultured under standard incubation conditions (37 ºC, 5% CO2) and grown in RPMI supplemented with 5% FBS and 1μg/mL P/S
Extracted molecule total RNA
Extraction protocol Cells were harvested at 6 h using Versagene RNA purification and DNase treatment kits. Six wells of a 6-well plate were pooled for each biological replicate.
Label biotin
Label protocol 10ug of cRNA is reverse transcribed to make ss cDNA of the correct sense to hybridize to the probes on the array. A total of 5.5ug of this product is fragmented, and then labeled with Terminal Deoxy Transferase with Biotin
Hybridization protocol Arrays were hybridized as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
Scan protocol GeneChips were scanned using the The GeneChip Scanner as described in the GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual v4
Description 977DWW23
Data processing Signal intensities across microarrays were RMA normalized
Submission date Oct 20, 2008
Last update date Oct 22, 2008
Contact name David W Wright
Phone 615-322-2636
Fax 615-343-1234
Organization name Vanderbilt University
Department Chemistry
Street address SC Stevenson Center 7, Station B 351822
City Nashville
State/province TN
ZIP/Postal code 37235-1822
Country USA
Platform ID GPL6246
Series (1)
GSE13281 Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells

Data table header descriptions
VALUE RMA normalized data (log2)

Data table
10344614 8.88408
10344616 2.10597
10344618 2.71778
10344620 3.65834
10344622 6.86418
10344624 8.80827
10344633 9.58169
10344637 9.54882
10344653 3.8104
10344658 7.23842
10344674 3.04333
10344679 7.63131
10344705 4.45352
10344707 6.04557
10344713 9.52285
10344715 3.82223
10344717 3.83758
10344719 5.40334
10344721 2.27702
10344723 8.78836

Total number of rows: 28853

Table truncated, full table size 475 Kbytes.

Supplementary file Size Download File type/resource
GSM335280.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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