 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 25, 2019 |
| Title |
tonsil CD14+ cells |
| Sample type |
SRA |
| |
|
| Source name |
tonsil CD14+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
individual: donor 1
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3’ v2 Reagent Kit (10X Genomics) according to manufacturer’s protocol
Indexed sequencing libraries were constructed using the reagents from the Chromium Single Cell 3’ v2 Reagent Kit, following these steps: (1) fragmentation, end repair and A-tailing; (2) size selection with SPRI select; (3) adaptor ligation; (4) post ligation cleanup with SPRI select; (5) sample index PCR and cleanup with SPRI select beads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
single-cell RNA-seq
|
| Data processing |
quality control, Cell Ranger Single Cell Software Suite (v2.0.1)
sample de-multiplexing, Cell Ranger Single Cell Software Suite (v2.0.1)
barcode processing, Cell Ranger Single Cell Software Suite (v2.0.1)
single-cell 3′ gene counting , Cell Ranger Single Cell Software Suite (v2.0.1)
alignment, Cell Ranger Single Cell Software Suite (v2.0.1)
Genome_build: GRCh38
|
| |
|
| Submission date |
Sep 05, 2018 |
| Last update date |
Apr 25, 2019 |
| Contact name |
Paul Gueguen |
| E-mail(s) |
paul.gueguen@unil.ch
|
| Organization name |
Institut Curie
|
| Department |
Inserm U932
|
| Street address |
26 rue D'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE119506 |
Single-cell RNA-seq analysis of human tonsil CD14+ cells |
|
| Relations |
| BioSample |
SAMN09979284 |
| SRA |
SRX4643789 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3375767_barcodes.tsv.gz |
7.5 Kb |
(ftp)(http) |
TSV |
| GSM3375767_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSM3375767_matrix.mtx.gz |
9.8 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
