|
| Status |
Public on Sep 03, 2020 |
| Title |
N2 control |
| Sample type |
RNA |
| |
|
| Source name |
Control RNAi in whole body
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain/background: N2
tissue: whole body
RNAi: Control
|
| Treatment protocol |
N2, VP303, NR350 or TU3401 worms were subjected to control or ash-2 RNAi for 3 days from egg stage to young adult stage.
|
| Growth protocol |
All strains were maintained at 20 °C on nematode growth medium (NGM) plates seeded with Escherichia coli OP50 as previously described.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen) in N2, VP303, NR350, TU3401 worms under Control RNAi and ash-2 RNAi conditions.
|
| Label |
biotin
|
| Label protocol |
Ambion WT cDNA Expression Kit was used according to the protocol.
|
| |
|
| Hybridization protocol |
Fragmented and labelled cDNA was hybridised to GeneChip C. elegans Gene 1.0 ST Array (Affymetrix).
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
The probe set signals were calculated with the robust multi-array average (RMA) algorithm implemented in GeneSpring GX 11.0.2 (Agilent Technologies).
|
| |
|
| Submission date |
Sep 13, 2018 |
| Last update date |
Sep 05, 2020 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19230 |
| Series (1) |
| GSE119917 |
Intestine-to-germline transmission of epigenetic information transgenerationally ensures systemic stress resistance in C. elegans |
|
