|
| Status |
Public on Sep 28, 2018 |
| Title |
Confront Water 1 |
| Sample type |
RNA |
| |
|
| Source name |
1 day after confrontation
|
| Organism |
Mus musculus |
| Characteristics |
background strain: AKR/J (however, it may also have been crossed with ddY or other lines).
group: Confront Water
tissue: brain hippocampus
|
| Treatment protocol |
Two mice were housed in the partitioned cage for 1 month to establish territorial consciousness (single housing); then the partition was removed to expose the mice to the confrontational stress, and subsequently, the two mice cohabited in the same cage (confrontational housing).
|
| Growth protocol |
Mice at 4 weeks of age were housed in a group of six in a cage for 5 days to habituate them to novel conditions. The mice were then divided into two groups, namely confrontationally and group housed. In brief, for confrontational housing, a standard polycarbonate cage was divided into two identical subunits by a stainless-steel partition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the hippocampus using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Total RNA was processed to synthesize biotinylated cRNA using One-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array.
|
| Scan protocol |
GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Description |
3 month
|
| Data processing |
Normalized by using Supernorm (Skylight-biotech); https://www.ncbi.nlm.nih.gov/pubmed/14718068
|
| |
|
| Submission date |
Sep 27, 2018 |
| Last update date |
Sep 28, 2018 |
| Contact name |
Tomokazu Konishi |
| E-mail(s) |
konishi@akita-pu.ac.jp
|
| Phone |
+81-18-872-1603
|
| Organization name |
Akita Prefectural University
|
| Department |
Bioresource Sciences
|
| Lab |
Molecular Genetics
|
| Street address |
Shimoshinjyo Nishi
|
| City |
Akita |
| State/province |
Akita |
| ZIP/Postal code |
010-0195 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE120546 |
Psychosocial stress-induced brain atrophy and its suppression via theanine intake |
|
