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Sample GSM3402798 Query DataSets for GSM3402798
Status Public on Sep 28, 2018
Title Confront Water 1
Sample type RNA
Source name 1 day after confrontation
Organism Mus musculus
Characteristics background strain: AKR/J (however, it may also have been crossed with ddY or other lines).
group: Confront Water
tissue: brain hippocampus
Treatment protocol Two mice were housed in the partitioned cage for 1 month to establish territorial consciousness (single housing); then the partition was removed to expose the mice to the confrontational stress, and subsequently, the two mice cohabited in the same cage (confrontational housing).
Growth protocol Mice at 4 weeks of age were housed in a group of six in a cage for 5 days to habituate them to novel conditions. The mice were then divided into two groups, namely confrontationally and group housed. In brief, for confrontational housing, a standard polycarbonate cage was divided into two identical subunits by a stainless-steel partition.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the hippocampus using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
Label biotin
Label protocol Total RNA was processed to synthesize biotinylated cRNA using One-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA, USA)
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array.
Scan protocol GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Description 3 month
Data processing Normalized by using Supernorm (Skylight-biotech);
Submission date Sep 27, 2018
Last update date Sep 28, 2018
Contact name Tomokazu Konishi
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
Platform ID GPL16570
Series (1)
GSE120546 Psychosocial stress-induced brain atrophy and its suppression via theanine intake

Data table header descriptions
VALUE z-score

Data table
17200001 0.586740332
17200003 0.267541103
17200005 0.0788663
17200007 0.28703252
17200009 0.157854627
17200011 -0.007694067
17200013 0.224096033
17200015 0.764181597
17200019 0.116954985
17200021 0.300861942
17200023 0.465997021
17200025 -0.05386781
17200027 0.240995631
17200029 0.240614548
17200031 0.233619984
17200033 0.573508287
17200035 -0.084961766
17200037 -0.147732041
17200039 0.892422268

Total number of rows: 41345

Table truncated, full table size 816 Kbytes.

Supplementary file Size Download File type/resource
GSM3402798_701035-001_MoGene-2_0-st_.CEL.gz 8.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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