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Sample GSM3417351 Query DataSets for GSM3417351
Status Public on Feb 16, 2019
Title trained_+4h_Ex_4
Sample type SRA
 
Source name muscle vastus lateralis
Organism Homo sapiens
Characteristics tissue: skeletal muscle
individual: 4
age: 21
training status: trained
time point: +4 h after exercise
exercised/non-exercised muscle: exercised (Ex)
Sex: male
Treatment protocol An exercise session (60 min, one-legged knee extension, ~65% of maximal power in the one-legged ramp test) started 2 h 35 min after a standardized breakfast. The participants ate a standardized lunch 1 h 20 min after the exercise session. This test session was repeated after an aerobic training programme (2 month, 1 h/day, 5 sessions/week) comprised training sessions on cycle ergometer (60 min) that alternated between continuous and intermittent exercise.
Extracted molecule polyA RNA
Extraction protocol Samples from the vastus lateralis muscle were taken using a microbiopsy technique and were frozen in liquid nitrogen; RNA was extracted from the samples using an RNeasy mini kit (Qiagen) with DNase treatment (Fermentas, Lithuania)
Libraries were constructed from 300 ng of RNA using a NEB Next Ultra II RNA kit. Poly-A mRNA was isolated using magnetic particles associated with 24-dT oligonucleotides by means of NEB Next Poly(A) mRNA Magnetic Isolation Module.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description 5PR3_S24_L004
Data processing Quality control and trimming were performed using Trimmomatic v0.36
High-quality reads were mapped to the reference human genome GRCh38 and exon–exon junctions were identified using HISAT v2.1.0
Reads were counted for known exons of each gene using HTseq v0.6.1
Normalization of read counts provided by DESeq method
Genome_build: GRCh38
Supplementary_files_format_and_content: Two tab-delimited text files include normalized read counts and FPKM for each sample
 
Submission date Oct 04, 2018
Last update date Feb 16, 2019
Contact name Pavel Makhnovsky
E-mail(s) maxpauel@gmail.com
Organization name Institute of Biomedical Problems of the Russian Academy of Sciences
Lab Laboratory of muscle physiology
Street address 76A Khoroshevskoye shosse
City Moscow
ZIP/Postal code 123007
Country Russia
 
Platform ID GPL16791
Series (1)
GSE120862 Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle
Relations
BioSample SAMN10180078
SRA SRX4801242

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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