|
| Status |
Public on Nov 07, 2018 |
| Title |
IL-10- CIR rich splenocytes 1 |
| Sample type |
SRA |
| |
|
| Source name |
Splenic T-cells
|
| Organism |
Mus musculus |
| Characteristics |
phenotype: rich
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using TRIzol LS reagent (Life Technology)
sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
log.norm.counts.txt
|
| Data processing |
low quality bases were trimmed with Trimmomatic 0.33 (parameters: LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36)
Alignment and summarization were done using Tophat and HTSeq count
Genome_build: GRCm38.84
Supplementary_files_format_and_content: tab-delimited text file containing log2 normalized counts for all samples provided by DESeq2
|
| |
|
| Submission date |
Oct 15, 2018 |
| Last update date |
Nov 07, 2018 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
|
| Street address |
Martinistrasse 52
|
| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE121284 |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse Bulk-seq] |
| GSE121393 |
Molecular and functional heterogeneity of IL-10-producing CD4+ T cells |
|
| Relations |
| BioSample |
SAMN10245245 |
| SRA |
SRX4890488 |
