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Status
Public on Nov 26, 2008
Title
CIS_baseline_rep1_cis006
Sample type
RNA
Source name
CD4+ Tcells
Organism
Homo sapiens Characteristics
naïve CD4+ Tcells
Treatment protocol
Untouched naïve CD4+ T cells were isolated by negative selection using Dynabeads® magnetic technology (Invitrogen, Carlsbad, CA). CD4+ T cells purity was assessed by FACS analysis (> 95%, data not shown)
Growth protocol
Peripheral blood mononuclear cells (PBMC) were separated in a ficoll gradient and frozen in liquid nitrogen until ready for CD4+ isolation
Extracted molecule
total RNA
Extraction protocol
RNA was then extracted using RNeasy® Mini kit (Quiagen, Valencia, CA), amplified with MessageAmpTM II aRNA kit 20 (Ambion, Austin, TX)
Label
Biotin
Label protocol
Affymetrix protocol
Hybridization protocol
hybridization onto Affymetrix® Human Genome U133 Plus2.0 arrays (NIH Neuroscience Microarray Consortium, Phoenix, AZ).
Scan protocol
Affymetrix protocol
Description
cis006-HGU133_2
Data processing
Normalized in BRBArrayTools using almostRMA, using default affymterix setting chosen by the program. There was no other scaling or trimming.
Submission date
Nov 25, 2008
Last update date
Aug 28, 2018
Contact name
Sergio Baranzini
E-mail(s)
sebaran@cgl.ucsf.edu Organization name
University of California San Francisco
Department
Neurology
Lab
Baranzini
Street address
513 Parnassus Ave
City
San Francisco
State/province
CA
ZIP/Postal code
94143
Country
USA
Platform ID
GPL570 Series (1)
GSE13732 CIS (multiple sclerosis) (case-control) (time-series)
Relations Reanalyzed by
GSE119087
Data table header descriptions ID_REF VALUE AlmostRMA (implemented in BRBArrayTools)
Data table ID_REF VALUE 1007_s_at
6.886018753
1053_at
5.121418953
117_at
6.233316898
121_at
7.474121094
1255_g_at
2.620640755
1294_at
10.00532341
1316_at
4.817406178
1320_at
3.161184549
1405_i_at
10.47194099
1431_at
4.34276104
1438_at
4.149931908
1487_at
7.285375595
1494_f_at
5.551700592
1552256_a_at
6.154998302
1552257_a_at
6.635351181
1552258_at
5.423444271
1552261_at
4.289989471
1552263_at
7.501505375
1552264_a_at
6.587303162
1552266_at
2.345538139
54675 1212 Kbytes .Supplementary file Size Download File type/resource GSM345082.CEL.gz
4.5 Mb
(ftp) (http) CEL
Processed data included within Sample table
