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Status
Public on Nov 26, 2008
Title
CIS_baseline_rep1_cis015
Sample type
RNA
Source name
CD4+ Tcells
Organism
Homo sapiens Characteristics
naïve CD4+ Tcells
Treatment protocol
Untouched naïve CD4+ T cells were isolated by negative selection using Dynabeads® magnetic technology (Invitrogen, Carlsbad, CA). CD4+ T cells purity was assessed by FACS analysis (> 95%, data not shown)
Growth protocol
Peripheral blood mononuclear cells (PBMC) were separated in a ficoll gradient and frozen in liquid nitrogen until ready for CD4+ isolation
Extracted molecule
total RNA
Extraction protocol
RNA was then extracted using RNeasy® Mini kit (Quiagen, Valencia, CA), amplified with MessageAmpTM II aRNA kit 20 (Ambion, Austin, TX)
Label
Biotin
Label protocol
Affymetrix protocol
Hybridization protocol
hybridization onto Affymetrix® Human Genome U133 Plus2.0 arrays (NIH Neuroscience Microarray Consortium, Phoenix, AZ).
Scan protocol
Affymetrix protocol
Description
cis015-HGU133_2
Data processing
Normalized in BRBArrayTools using almostRMA, using default affymterix setting chosen by the program. There was no other scaling or trimming.
Submission date
Nov 25, 2008
Last update date
Aug 28, 2018
Contact name
Sergio Baranzini
E-mail(s)
sebaran@cgl.ucsf.edu Organization name
University of California San Francisco
Department
Neurology
Lab
Baranzini
Street address
513 Parnassus Ave
City
San Francisco
State/province
CA
ZIP/Postal code
94143
Country
USA
Platform ID
GPL570 Series (1)
GSE13732 CIS (multiple sclerosis) (case-control) (time-series)
Relations Reanalyzed by
GSE119087
Data table header descriptions ID_REF VALUE AlmostRMA (implemented in BRBArrayTools)
Data table ID_REF VALUE 1007_s_at
7.139511585
1053_at
5.144817829
117_at
4.405593395
121_at
7.290803432
1255_g_at
2.45126915
1294_at
9.491874695
1316_at
4.914991856
1320_at
3.516951561
1405_i_at
9.468515396
1431_at
4.282876968
1438_at
4.21622467
1487_at
6.744447231
1494_f_at
5.601429462
1552256_a_at
6.298581123
1552257_a_at
7.78610611
1552258_at
4.907238483
1552261_at
4.227180958
1552263_at
8.474849701
1552264_a_at
6.44101429
1552266_at
3.749311209
54675 1212 Kbytes .Supplementary file Size Download File type/resource GSM345093.CEL.gz
4.6 Mb
(ftp) (http) CEL
Processed data included within Sample table
