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Status
Public on Nov 26, 2008
Title
CIS_baseline_rep1_cis016_2
Sample type
RNA
Source name
CD4+ Tcells
Organism
Homo sapiens Characteristics
naïve CD4+ Tcells
Treatment protocol
Untouched naïve CD4+ T cells were isolated by negative selection using Dynabeads® magnetic technology (Invitrogen, Carlsbad, CA). CD4+ T cells purity was assessed by FACS analysis (> 95%, data not shown)
Growth protocol
Peripheral blood mononuclear cells (PBMC) were separated in a ficoll gradient and frozen in liquid nitrogen until ready for CD4+ isolation
Extracted molecule
total RNA
Extraction protocol
RNA was then extracted using RNeasy® Mini kit (Quiagen, Valencia, CA), amplified with MessageAmpTM II aRNA kit 20 (Ambion, Austin, TX)
Label
Biotin
Label protocol
Affymetrix protocol
Hybridization protocol
hybridization onto Affymetrix® Human Genome U133 Plus2.0 arrays (NIH Neuroscience Microarray Consortium, Phoenix, AZ).
Scan protocol
Affymetrix protocol
Description
cis016-HGU133_2_2
Data processing
Normalized in BRBArrayTools using almostRMA, using default affymterix setting chosen by the program. There was no other scaling or trimming.
Submission date
Nov 25, 2008
Last update date
Aug 28, 2018
Contact name
Sergio Baranzini
E-mail(s)
sebaran@cgl.ucsf.edu Organization name
University of California San Francisco
Department
Neurology
Lab
Baranzini
Street address
513 Parnassus Ave
City
San Francisco
State/province
CA
ZIP/Postal code
94143
Country
USA
Platform ID
GPL570 Series (1)
GSE13732 CIS (multiple sclerosis) (case-control) (time-series)
Relations Reanalyzed by
GSE119087
Data table header descriptions ID_REF VALUE AlmostRMA (implemented in BRBArrayTools)
Data table ID_REF VALUE 1007_s_at
6.920860291
1053_at
4.91629076
117_at
5.045212746
121_at
7.102874756
1255_g_at
2.658664465
1294_at
9.57459259
1316_at
5.082657337
1320_at
3.552863359
1405_i_at
9.852513313
1431_at
3.994944096
1438_at
4.411554813
1487_at
6.830395222
1494_f_at
5.335781574
1552256_a_at
6.086224556
1552257_a_at
6.8301754
1552258_at
5.648504257
1552261_at
4.697489738
1552263_at
9.277028084
1552264_a_at
7.2309618
1552266_at
2.519771099
54675 1211 Kbytes .Supplementary file Size Download File type/resource GSM345094.CEL.gz
4.4 Mb
(ftp) (http) CEL
Processed data included within Sample table
