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Sample GSM3465072 Query DataSets for GSM3465072
Status Public on Nov 10, 2018
Title WT_rep2_neuropathology
Sample type RNA
 
Source name WT_neuropathology
Organism Mus musculus
Characteristics strain background: C57BL/6J
age: 8 weeks
genotype/variation: wild type
tissue: Olfactory epithelium
cell type: Olfactory sensory neuron
Extracted molecule total RNA
Extraction protocol Olfactory epithelial tissue from two wild-type and two MeCP2 null mice were dissected. Total RNAs were isolated from the olfactory epithelial tissue using TRIZOL reagent (Invitrogen).
Label Nanostring
Label protocol manufacturer's protocol
 
Hybridization protocol 100ng of total RNA was hybridized to neuropathology panel (XT-CSO-MNROP1-12) using manufacturer's protocol
Scan protocol Hybridized panels were scanned using nCounter Analyzer
Description WT re2
Data processing The gene expression levels of the two biological replicates showed a strong correlation (r = 0.997 of WT, r= 0.997 of Mecp2 KO) and combined for downstream analysis. Using nSolver ver4.0, gene expression values in nCounter Mouse Neuropathology Panel Kit were normalized with positive ERCC spike-in and reference gene, Abl1.
 
Submission date Nov 09, 2018
Last update date Nov 10, 2018
Contact name Wooje Lee
E-mail(s) ntinamu001@gmail.com
Organization name Chosun University
Department Cellular and Molecular Medicine
Street address 309, Pilmun-daero, Dong-gu
City Gwangju
ZIP/Postal code 61452
Country South Korea
 
Platform ID GPL25789
Series (2)
GSE122365 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [NanoString]
GSE122366 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
3110043O21Rik 505.82
AI464131 92.6
Abat 269.84
Abl1 196.16
Acaa1a 429.15
Ache 37.84
Acin1 1830.12
Actn1 1098.27
Acvrl1 34.85
Adam10 792.59
Adcy5 7.97
Adcy8 31.86
Adcy9 128.45
Adcyap1 16.93
Adora1 19.91
Adora2a 7.97
Adra2a 177.24
Adrb2 100.57
Ager 22.9
Aif1 10.95

Total number of rows: 784

Table truncated, full table size 9 Kbytes.




Supplementary file Size Download File type/resource
GSM3465072_WT_rep2_neuropathology.RCC.gz 9.0 Kb (ftp)(http) RCC
Processed data included within Sample table
Processed data are available on Series record

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