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Sample GSM3465073 Query DataSets for GSM3465073
Status Public on Nov 10, 2018
Title Mecp2KO_rep1_neuropathology
Sample type RNA
 
Source name Mecp2KO_neuropathology
Organism Mus musculus
Characteristics strain background: C57BL/6J
age: 8 weeks
genotype/variation: Mecp2KO
tissue: Olfactory epithelium
cell type: Olfactory sensory neuron
Extracted molecule total RNA
Extraction protocol Olfactory epithelial tissue from two wild-type and two MeCP2 null mice were dissected. Total RNAs were isolated from the olfactory epithelial tissue using TRIZOL reagent (Invitrogen).
Label Nanostring
Label protocol manufacturer's protocol
 
Hybridization protocol 100ng of total RNA was hybridized to neuropathology panel (XT-CSO-MNROP1-12) using manufacturer's protocol
Scan protocol Hybridized panels were scanned using nCounter Analyzer
Description KO re1
Data processing The gene expression levels of the two biological replicates showed a strong correlation (r = 0.997 of WT, r= 0.997 of Mecp2 KO) and combined for downstream analysis. Using nSolver ver4.0, gene expression values in nCounter Mouse Neuropathology Panel Kit were normalized with positive ERCC spike-in and reference gene, Abl1.
 
Submission date Nov 09, 2018
Last update date Nov 10, 2018
Contact name Wooje Lee
E-mail(s) ntinamu001@gmail.com
Organization name Chosun University
Department Cellular and Molecular Medicine
Street address 309, Pilmun-daero, Dong-gu
City Gwangju
ZIP/Postal code 61452
Country South Korea
 
Platform ID GPL25789
Series (2)
GSE122365 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [NanoString]
GSE122366 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
3110043O21Rik 568.85
AI464131 87.62
Abat 239.31
Abl1 196.16
Acaa1a 555.77
Ache 47.08
Acin1 2004.7
Actn1 1224.01
Acvrl1 53.62
Adam10 757.16
Adcy5 9.15
Adcy8 24.85
Adcy9 155.62
Adcyap1 7.85
Adora1 11.77
Adora2a 5.23
Adra2a 241.92
Adrb2 108.54
Ager 19.62
Aif1 11.77

Total number of rows: 784

Table truncated, full table size 9 Kbytes.




Supplementary file Size Download File type/resource
GSM3465073_Mecp2KO_rep1_neuropathology.RCC.gz 8.9 Kb (ftp)(http) RCC
Processed data included within Sample table
Processed data are available on Series record

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