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Sample GSM3465074 Query DataSets for GSM3465074
Status Public on Nov 10, 2018
Title Mecp2KO_rep2_neuropathology
Sample type RNA
 
Source name Mecp2KO_neuropathology
Organism Mus musculus
Characteristics strain background: C57BL/6J
age: 8 weeks
genotype/variation: Mecp2KO
tissue: Olfactory epithelium
cell type: Olfactory sensory neuron
Extracted molecule total RNA
Extraction protocol Olfactory epithelial tissue from two wild-type and two MeCP2 null mice were dissected. Total RNAs were isolated from the olfactory epithelial tissue using TRIZOL reagent (Invitrogen).
Label Nanostring
Label protocol manufacturer's protocol
 
Hybridization protocol 100ng of total RNA was hybridized to neuropathology panel (XT-CSO-MNROP1-12) using manufacturer's protocol
Scan protocol Hybridized panels were scanned using nCounter Analyzer
Description KO re2
Data processing The gene expression levels of the two biological replicates showed a strong correlation (r = 0.997 of WT, r= 0.997 of Mecp2 KO) and combined for downstream analysis. Using nSolver ver4.0, gene expression values in nCounter Mouse Neuropathology Panel Kit were normalized with positive ERCC spike-in and reference gene, Abl1.
 
Submission date Nov 09, 2018
Last update date Nov 10, 2018
Contact name Wooje Lee
E-mail(s) ntinamu001@gmail.com
Organization name Chosun University
Department Cellular and Molecular Medicine
Street address 309, Pilmun-daero, Dong-gu
City Gwangju
ZIP/Postal code 61452
Country South Korea
 
Platform ID GPL25789
Series (2)
GSE122365 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [NanoString]
GSE122366 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
3110043O21Rik 693.22
AI464131 84.21
Abat 221.83
Abl1 196.16
Acaa1a 438.52
Ache 28.76
Acin1 1992.36
Actn1 1070.12
Acvrl1 45.19
Adam10 817.48
Adcy5 7.19
Adcy8 22.59
Adcy9 137.62
Adcyap1 16.43
Adora1 12.32
Adora2a 5.13
Adra2a 205.4
Adrb2 133.51
Ager 18.49
Aif1 8.22

Total number of rows: 784

Table truncated, full table size 9 Kbytes.




Supplementary file Size Download File type/resource
GSM3465074_Mecp2KO_rep2_neuropathology.RCC.gz 9.0 Kb (ftp)(http) RCC
Processed data included within Sample table
Processed data are available on Series record

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