NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM348010 Query DataSets for GSM348010
Status Public on Dec 06, 2008
Title hESC-CM-2
Sample type RNA
 
Channel 1
Source name hESC-CM-2
Organism Homo sapiens
Characteristics Cardiomyocytes that were differentiated then purified from hESCs
Extracted molecule total RNA
Extraction protocol Total RNA was isolated as described previously in Trizol (Invitrogen) followed by purification over a Qiagen RNeasy column (Qiagen).
Label Cy5
Label protocol Using Low RNA Input Fluorescent Linear Amplification Kits (Agilent Technologies, Santa Clara, CA, USA), cDNA was reverse transcribed from each RNA sample, and cRNA was then transcribed and fluorescently labeled from each cDNA sample.
 
Channel 2
Source name Pooled Reference
Organism Homo sapiens
Characteristics Pooled RNA from undifferentiated and differentiated hESCs
Extracted molecule total RNA
Extraction protocol Total RNA was isolated as described previously in Trizol (Invitrogen) followed by purification over a Qiagen RNeasy column (Qiagen).
Label Cy3
Label protocol Using Low RNA Input Fluorescent Linear Amplification Kits (Agilent Technologies, Santa Clara, CA, USA), cDNA was reverse transcribed from each RNA sample, and cRNA was then transcribed and fluorescently labeled from each cDNA sample.
 
 
Hybridization protocol 825 ng of Cy3- and Cy5- labeled and amplified cRNA was mixed and fragmented according to the Agilent technology protocol. cRNA was hybridized to 4x44K whole human genome microarray slides from Agilent (Part G4112F) according to the manufacturer’s instructions. The hybridization was carried in a rotating hybridization chamber in the dark at 65°C for 17 h.
Scan protocol The array was scanned using Agilent G2505B DNA microarray scanner.
Description hESC-CM-2
Data processing The image files were extracted using Agilent Feature Extraction software version 9.5.1 applying LOWESS background subtraction and dye-normalization.
 
Submission date Dec 04, 2008
Last update date Dec 05, 2008
Contact name Kitchener D. Wilson
E-mail(s) kitchwilson@stanford.edu
Organization name Stanford University
Street address S140 Grant Bldg
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL4133
Series (1)
GSE13834 Transcriptional Profiling of Human Embryonic Stem Cell-Derived Cardiomyocytes

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio (rProcessedSignal/gProcessedSignal)
LogRatioError
PValueLogRatio
gProcessedSignal
rProcessedSignal

Data table
ID_REF VALUE LogRatioError PValueLogRatio gProcessedSignal rProcessedSignal
1 1.35E-03 6.14E-02 9.82E-01 4.84E+04 4.85E+04
2 0.00E+00 6.53E-01 1.00E+00 9.85E+00 5.53E+00
3 0.00E+00 6.53E-01 1.00E+00 9.98E+00 5.60E+00
4 0.00E+00 6.52E-01 1.00E+00 1.01E+01 5.67E+00
5 0.00E+00 6.52E-01 1.00E+00 1.02E+01 5.74E+00
6 0.00E+00 6.52E-01 1.00E+00 1.03E+01 5.80E+00
7 0.00E+00 6.52E-01 1.00E+00 1.04E+01 5.86E+00
8 0.00E+00 6.52E-01 1.00E+00 1.05E+01 5.91E+00
9 0.00E+00 6.52E-01 1.00E+00 1.05E+01 5.96E+00
10 0.00E+00 6.51E-01 1.00E+00 1.06E+01 6.01E+00
11 0.00E+00 6.51E-01 1.00E+00 1.07E+01 6.04E+00
12 3.70E-01 7.04E-02 1.46E-07 5.87E+02 1.38E+03
13 0.00E+00 6.51E-01 1.00E+00 1.08E+01 6.11E+00
14 -7.00E-02 6.23E-02 2.62E-01 6.67E+02 5.68E+02
15 1.38E-01 3.34E-01 6.79E-01 1.42E+01 1.95E+01
16 2.95E-02 6.15E-02 6.31E-01 1.48E+04 1.58E+04
17 4.35E-01 7.76E-02 2.06E-08 1.23E+02 3.34E+02
18 -2.36E-01 6.80E-02 5.24E-04 3.55E+02 2.06E+02
19 2.81E-01 6.66E-02 2.40E-05 3.25E+04 6.22E+04
20 -1.02E-01 3.82E-01 7.90E-01 1.64E+01 1.30E+01

Total number of rows: 45015

Table truncated, full table size 2249 Kbytes.




Supplementary file Size Download File type/resource
GSM348010.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap