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Sample GSM348312 Query DataSets for GSM348312
Status Public on Jul 22, 2009
Title WT-OVX-E2-3
Sample type other
 
Source name uterus from ovariectomized and E2 treated wild type mouse
Organism Mus musculus
Characteristics Strain: C57BL/6
Gender: female
Tissue: the uterus
Treatment protocol At 15 week old, female mice were ovariectomized, and after one week, estradiol (E2) was delivered at a concentration of 0.050 mg/kg body weight/day. 24 hours after chemical treatment, uteruses were dissected.
Extracted molecule other
Extraction protocol To isolate miRNA fractions from total RNA, 100μg total RNA was fractionated and cleaned up with the flashPAGETM fractionator System and reagents (Ambion) per the manufacturer’s recommendation.
Label Cy5
Label protocol mirVanaTM miRNA Labeling Kit (Ambion) and amine-reactive dyes as recommended by the manufacturer.
 
Hybridization protocol A 3x miRNA Hybridization Buffer (Ambion) was added to the fluorescently labeled miRNAs and the solution was heated at 95ºC for 3 min. Slides were hybridized form 12 to 16 h at 42 ºC in sealed cassettes using a water bath. Following hybridization, the slides were washed and dried prior to a high-resolution scan.
Scan protocol Scan on a GenePix 4000B (Axon Instruments, CA, USA). Raw data were normalized and analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA).
Description E2 treatment of ovariectomized wild type mouse
Data processing We used Array-Pro Analyzer Ver4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) to determine the signal intensity of each spot and its local background on microarrays. We calculated net intensity by subtracting the mean intensity of all pixels within the local background area from the mean intensity of all pixels within the spot areas.
 
Submission date Dec 08, 2008
Last update date Jul 22, 2009
Contact name kaoru yamagata
E-mail(s) ykaoru7@hotmail.com
Organization name university of tokyo
Street address yayoi 1-1-1
City bukyo-ku
State/province Tokyo
ZIP/Postal code 113-0032
Country Japan
 
Platform ID GPL5105
Series (2)
GSE13858 Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment
GSE14031 Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

Data table header descriptions
ID_REF
VALUE Net Intensity after Normalization

Data table
ID_REF VALUE
PS20108 151.7966553
PS20132 121.0488293
PS20156 118.858863
PS20180 132.9070233
PS20106 144.5618727
PS20130 130.018729
PS20154 132.5137127
PS20178 111.8956523
PS20104 130.8949833
PS20128 123.677592
PS20152 136.832107
PS20176 137.81806
PS20102 128.2153847
PS20126 119.5010033
PS20150 126.536455
PS20174 140.2809363
PS20124 148.5404683
PS20148 122.0923077
PS20172 123.2428093
PS20122 134.084281

Total number of rows: 378

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM348312.jpg.gz 5.3 Mb (ftp)(http) JPG
GSM348312.txt.gz 6.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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