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Sample GSM348317 Query DataSets for GSM348317
Status Public on Jul 22, 2009
Title WT-OVX-E2-1
Sample type other
Source name uterus from ovariectomized and E2 treated wild type mouse
Organism Mus musculus
Characteristics Strain: C57BL/6
Gender: female
Tissue: the uterus
Treatment protocol At 15 week old, female mice were ovariectomized, and after one week, estradiol (E2) was delivered at a concentration of 0.050 mg/kg body weight/day. 24 hours after chemical treatment, uteruses were dissected.
Growth protocol 16 weeks old
Extracted molecule other
Extraction protocol To isolate miRNA fractions from total RNA, 100μg total RNA was fractionated and cleaned up with the flashPAGETM fractionator System and reagents (Ambion) per the manufacturer’s recommendation.
Label Cy5
Label protocol mirVanaTM miRNA Labeling Kit (Ambion) and amine-reactive dyes as recommended by the manufacturer.
Hybridization protocol A 3x miRNA Hybridization Buffer (Ambion) was added to the fluorescently labeled miRNAs and the solution was heated at 95ºC for 3 min. Slides were hybridized form 12 to 16 h at 42 ºC in sealed cassettes using a water bath. Following hybridization, the slides were washed and dried prior to a high-resolution scan.
Scan protocol Scan on a GenePix 4000B (Axon Instruments, CA, USA). Raw data were normalized and analyzed using Array-Pro Analyzer Version 4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA).
Description E2 treatment of ovariectomized wild type mouse
Data processing We used Array-Pro Analyzer Ver4.5 (Media Cybernetics, Inc., Silver Spring, MD, USA) to determine the signal intensity of each spot and its local background on microarrays. We calculated net intensity by subtracting the mean intensity of all pixels within the local background area from the mean intensity of all pixels within the spot areas.
Submission date Dec 08, 2008
Last update date Jul 22, 2009
Contact name kaoru yamagata
Organization name university of tokyo
Street address yayoi 1-1-1
City bukyo-ku
State/province Tokyo
ZIP/Postal code 113-0032
Country Japan
Platform ID GPL5106
Series (2)
GSE13858 Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment
GSE14031 Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

Data table header descriptions
VALUE Net Intensity after Normalization

Data table
BM10004 190.096116
BM10017 149.6673305
BM10019 166.1746313
BM10022 165.8358163
BM10030 173.2443215
BM10032 151.9197885
BM10034 145.393338
BM10035 158.9838005
BM10048 4239.845624
BM10049 152.362709
BM10050 3839.9794
BM10053 192.4507868
BM10056 451.226057
BM10057 175.3074975
BM10063 161.1927235
BM10064 153.5627583
BM10065 147.8521143
BM10067 185.967871
BM10070 181.756342
BM10071 154.989946

Total number of rows: 662

Table truncated, full table size 12 Kbytes.

Supplementary file Size Download File type/resource
GSM348317.jpg.gz 2.5 Mb (ftp)(http) JPG
GSM348317.txt.gz 15.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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