|
Status |
Public on Nov 21, 2018 |
Title |
Sae2 G1 + CPT IP |
Sample type |
SRA |
|
|
Source name |
TP3352 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sae2::KMX
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
immunoprecipitation: Flag-tagged Sae2 phase: G1 genotype/variation: TP3352 MATa his3delta1 leu2delta0 met15delta0 ura3delta0 sae2::KMX treatment: camptothecin (100 uM)
|
Treatment protocol |
see growth protocol
|
Growth protocol |
alpha factor synchronization then either held in G1 or released into growth for 50 min., with or without camptothecin (100 uM)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
see BioRxiv https://doi.org/10.1101/394924 NEBNext DNA Library prep kit for Illumina (E6040S)
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
Lib 42
|
Data processing |
alignment with BWA-MEM duplicatemarking:PicardMarkDuplicates QC:samtoolsflagstat,idxstats Peakcalling:macs2 Genome_build: saccer3 Supplementary_files_format_and_content: bam files
|
|
|
Submission date |
Nov 20, 2018 |
Last update date |
Nov 21, 2018 |
Contact name |
Tanya T. Paull |
E-mail(s) |
tpaull@utexas.edu
|
Phone |
5122327803
|
Organization name |
Univ. of Texas at Austin
|
Department |
Molecular Biosciences
|
Lab |
Paull
|
Street address |
2500 Speedway MBB 2.448
|
City |
Austin |
State/province |
TX |
ZIP/Postal code |
78712 |
Country |
USA |
|
|
Platform ID |
GPL19756 |
Series (1) |
|
Relations |
BioSample |
SAMN10457068 |
SRA |
SRX5041899 |