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Sample GSM3486709 Query DataSets for GSM3486709
Status Public on Feb 12, 2019
Title Hdac1&2 cKO rep2
Sample type SRA
Source name cortex
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: cortex
age: embryonic day 12.5
Extracted molecule total RNA
Extraction protocol Cortex were removed on ice, and RNA was harvested using Trizol reagent.
TruSeq RNA Library Prep Kit
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Data processing Base calling was performed using bcl2fastq v1.8.4
Adapter trimming and quality filtering were performed using trim_gaplore v0.4.4
Alignment to the reference genome was performed using STAR v2.5.4b
Read counting was performed using featureCounts v1.6.1
Differential expression analysis was performed using R v3.4.1 and the R package DESeq2 v1.22.1
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited spreadsheet, FPKM values
Submission date Nov 21, 2018
Last update date Jul 06, 2019
Contact name Yunli Xie
Organization name Fudan University
Department Institutes of Brain Science
Lab Xie Lab
Street address 131 Dong'an Road, Shanghai
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
Platform ID GPL21273
Series (1)
GSE122834 Roles of HDAC1 and HDAC2 in the developing mouse brain
BioSample SAMN10461327
SRA SRX5054314

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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