|
Status |
Public on Feb 12, 2019 |
Title |
Hdac1&2 cKO rep3 |
Sample type |
SRA |
|
|
Source name |
cortex
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: cortex age: embryonic day 12.5
|
Extracted molecule |
total RNA |
Extraction protocol |
Cortex were removed on ice, and RNA was harvested using Trizol reagent. TruSeq RNA Library Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Base calling was performed using bcl2fastq v1.8.4 Adapter trimming and quality filtering were performed using trim_gaplore v0.4.4 Alignment to the reference genome was performed using STAR v2.5.4b Read counting was performed using featureCounts v1.6.1 Differential expression analysis was performed using R v3.4.1 and the R package DESeq2 v1.22.1 Genome_build: mm10 Supplementary_files_format_and_content: Tab-delimited spreadsheet, FPKM values
|
|
|
Submission date |
Nov 21, 2018 |
Last update date |
Jul 06, 2019 |
Contact name |
Yunli Xie |
Organization name |
Fudan University
|
Department |
Institutes of Brain Science
|
Lab |
Xie Lab
|
Street address |
131 Dong'an Road, Shanghai
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
|
|
Platform ID |
GPL21273 |
Series (1) |
GSE122834 |
Roles of HDAC1 and HDAC2 in the developing mouse brain |
|
Relations |
BioSample |
SAMN10461326 |
SRA |
SRX5054315 |