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Sample GSM351352 Query DataSets for GSM351352
Status Public on Dec 22, 2008
Title MTB/TRAS 0hr rep 1
Sample type RNA
 
Source name Mammary Gland uninduced
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS uninduced
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 4.52299138716018
100002_at 2.12611632349336
100003_at 2.51099026481454
100004_at 4.14987701674611
100005_at 5.23515585590891
100006_at 3.559789201276
100007_at 6.32290306209063
100009_r_at 2.98409620246388
100010_at 6.93751056218707
100011_at 7.3298649204058
100012_at 7.83594297640843
100013_at 6.91485569540779
100014_at 6.43833487281702
100015_at 5.37186925146341
100016_at 3.87118473091648
100017_at 2.19281029566567
100018_at 4.78479502610046
100019_at 6.83433082942171
100020_at 5.47455619052887
100021_at 3.54497188876652

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351352.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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