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Sample GSM351355 Query DataSets for GSM351355
Status Public on Dec 22, 2008
Title MTB/TRAS 24hr rep 1
Sample type RNA
 
Source name Mammary Gland 24hr Ras induction
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS induced for 24 hr
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 4.74463002456643
100002_at 2.18322879606632
100003_at 2.53843429270561
100004_at 4.21089451210407
100005_at 5.51708481937387
100006_at 3.7344554390578
100007_at 6.34482924128352
100009_r_at 3.14817881819583
100010_at 6.25826292539707
100011_at 5.95265760107725
100012_at 7.6434997945801
100013_at 6.63339010931333
100014_at 6.54477051280247
100015_at 5.76947157096718
100016_at 3.7448546889807
100017_at 2.23111844652467
100018_at 4.76916615177602
100019_at 8.0595189743197
100020_at 5.25265547030535
100021_at 3.68213579912153

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351355.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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