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Sample GSM351357 Query DataSets for GSM351357
Status Public on Dec 22, 2008
Title MTB/TRAS 24hr rep3
Sample type RNA
 
Source name Mammary Gland 24hr Ras induction
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS induced for 24 hr
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 4.863942247456
100002_at 2.15893043787781
100003_at 2.51685509480924
100004_at 4.2010863090047
100005_at 4.66863896677124
100006_at 3.88606797008533
100007_at 6.30019102545505
100009_r_at 3.07433699613314
100010_at 5.50779669039868
100011_at 5.89569258889682
100012_at 7.98696688892098
100013_at 6.80679669190427
100014_at 6.90534230468606
100015_at 5.8288990416767
100016_at 3.0943193662299
100017_at 2.21901086718145
100018_at 4.76280222463341
100019_at 8.42314745984206
100020_at 5.89746565117571
100021_at 3.61030202405613

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351357.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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