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Sample GSM351359 Query DataSets for GSM351359
Status Public on Dec 22, 2008
Title MTB/TRAS 48hr rep2
Sample type RNA
 
Source name Mammary Gland 48hr Ras induction
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS induced for 48hr
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 5.14818112217174
100002_at 2.14504840670908
100003_at 2.51041090302317
100004_at 4.19394485677121
100005_at 3.23161048039816
100006_at 3.83753966143353
100007_at 6.00997241198572
100009_r_at 3.19992267829948
100010_at 5.54297904678518
100011_at 6.13941937917799
100012_at 7.73138556161484
100013_at 7.39849631443875
100014_at 6.90550894081877
100015_at 5.96421809795112
100016_at 3.98720469853418
100017_at 2.19236260187942
100018_at 4.75835113603061
100019_at 8.88189090905286
100020_at 4.53367709593449
100021_at 3.70120754269638

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351359.CEL.gz 2.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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