|
Status |
Public on Dec 22, 2008 |
Title |
MTB/TRAS 48hr rep2 |
Sample type |
RNA |
|
|
Source name |
Mammary Gland 48hr Ras induction
|
Organism |
Mus musculus |
Characteristics |
Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
|
Treatment protocol |
Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
|
Growth protocol |
NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Affymetrix Generic Hybrdization
|
Scan protocol |
Arrays were scanned using the Affymetrix GeneArray Scanner
|
Description |
MTB/TRAS induced for 48hr
|
Data processing |
The data were analyzed with GCRMA using R and quantile normalized
|
|
|
Submission date |
Dec 16, 2008 |
Last update date |
Dec 22, 2008 |
Contact name |
Lewis A. Chodosh |
E-mail(s) |
chodosh@mail.med.upenn.edu
|
Fax |
215-573-6725
|
Organization name |
University of Pennsylvania
|
Department |
Cancer Biology
|
Lab |
Lewis Chodosh
|
Street address |
421 curie blvd, BRB II/III 616
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL81 |
Series (1) |
GSE13986 |
MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells |
|