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Sample GSM351362 Query DataSets for GSM351362
Status Public on Dec 22, 2008
Title MTB/TRAS 96hr rep2
Sample type RNA
 
Source name Mammary Gland 96hr Ras induction
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS induced for 96hr
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 4.27097388915686
100002_at 1.9485044197016
100003_at 2.33161754338422
100004_at 4.64705835406979
100005_at 5.62677731240494
100006_at 5.12992226126526
100007_at 6.29075982315229
100009_r_at 2.79725376143563
100010_at 6.51319187420506
100011_at 6.53912656716443
100012_at 8.78698912207302
100013_at 7.13405142775108
100014_at 6.39830494262623
100015_at 5.77833201193523
100016_at 5.92263222204604
100017_at 1.96953574637387
100018_at 4.49135800459396
100019_at 9.58588731326744
100020_at 6.55688234303928
100021_at 3.34334711732496

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351362.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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