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Sample GSM351364 Query DataSets for GSM351364
Status Public on Dec 22, 2008
Title MTB/TRAS 8d rep 1
Sample type RNA
 
Source name Mammary Gland 8d Ras induction
Organism Mus musculus
Characteristics Genotype: MTB/TRAS; Gender: Female; Tissue: Mammary gland; Strain: FVB
Treatment protocol Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Tumors were harvested at 1-cm
Growth protocol NMuMG, was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% bovine calf serum, 1% penicillin/streptomycin, and 2 mM L-glutamine.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Affymetrix Generic Hybrdization
Scan protocol Arrays were scanned using the Affymetrix GeneArray Scanner
Description MTB/TRAS induced for 8 day
Data processing The data were analyzed with GCRMA using R and quantile normalized
 
Submission date Dec 16, 2008
Last update date Dec 22, 2008
Contact name Lewis A. Chodosh
E-mail(s) chodosh@mail.med.upenn.edu
Fax 215-573-6725
Organization name University of Pennsylvania
Department Cancer Biology
Lab Lewis Chodosh
Street address 421 curie blvd, BRB II/III 616
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL81
Series (1)
GSE13986 MTB/TRAS short-term induction, tumor, and TGFbeta treated NMuMG cells

Data table header descriptions
ID_REF
VALUE Log2 transformed GCRMA

Data table
ID_REF VALUE
100001_at 4.48688760663266
100002_at 1.96122775749409
100003_at 2.29685555449464
100004_at 4.25692487526137
100005_at 6.03087318525683
100006_at 4.73147219537684
100007_at 6.57346181780284
100009_r_at 2.85888485681691
100010_at 6.10074805037894
100011_at 6.7784536985043
100012_at 9.78403047696348
100013_at 6.62152799297764
100014_at 6.89143955095926
100015_at 5.51628566814867
100016_at 5.8180610051226
100017_at 2.0329473778001
100018_at 4.5572565067216
100019_at 9.24227408136424
100020_at 6.6483198696432
100021_at 3.3933005182919

Total number of rows: 12488

Table truncated, full table size 328 Kbytes.




Supplementary file Size Download File type/resource
GSM351364.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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