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Sample GSM3521624 Query DataSets for GSM3521624
Status Public on Dec 02, 2019
Title Drosophila yakuba_zt3_25C
Sample type SRA
Source name RNA from fly heads
Organism Drosophila yakuba
Characteristics circadian timepoints: zt3
temperature: 25C
tissue: fly heads
Growth protocol Flies were grown at different temperatures and heads were collected at different temperatures.
Extracted molecule polyA RNA
Extraction protocol RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol. The precipitated RNAwas taken for library preparation.
Libraries were constructed Stranded ligation-based, total-RNA libraries preparation was modified from (Engreitz et al., 2013) as described in Reddy et al 2018, Biorxiv.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing quantification of tim isoforms looking manually the exon-exon juntion reads
Genome_build: dm3, droAnanas2, droSim1, droViri3, droYak2
Supplementary_files_format_and_content: table of tim isoform analysis; transcript quantification
Submission date Dec 19, 2018
Last update date Dec 02, 2019
Contact name Sebastian Kadener
Organization name Brandeis University
Street address 415 South Street
City Waltham
State/province MA
ZIP/Postal code 9190401
Country USA
Platform ID GPL19131
Series (2)
GSE124136 Differences in circadian rhythms in fly at different temperatures
GSE124142 Total RNA from different species fly heads
BioSample SAMN10616466
SRA SRX5167224

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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