NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3528288 Query DataSets for GSM3528288
Status Public on Dec 21, 2021
Title Rec12_pht1D
Sample type genomic
 
Channel 1
Source name FLAG ChIP DNA from rec12+-FLAG pht1 deletion cells harvested 5h after meiosis induction
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: pat1-114 rad50S rec12+-FLAG pht1 deletion
treatment: meiosis induction
chip antibody: anti-FLAG
Growth protocol Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested four or five hours after the induction.
Extracted molecule genomic DNA
Extraction protocol Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using anti-FLAG antibodies from Sigma-Aldrich.
Label biotin
Label protocol Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83).
 
Channel 2
Source name Input DNA from pat1-114 cells in the wild-type background harvested 4h after meiosis induction
Organism Schizosaccharomyces pombe
Characteristics genotype/variation: pat1-114 wild type
treatment: meiosis induction
chip antibody: None
Growth protocol Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested four or five hours after the induction.
Extracted molecule genomic DNA
Extraction protocol Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using anti-FLAG antibodies from Sigma-Aldrich.
Label biotin
Label protocol Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83).
 
 
Hybridization protocol 16 h at 45C using a hybridization oven 640 (Affymetrix) as described in the Affymetrix Chromatin Immunoprecipitation Assay Protocol (P/N 702238).
Scan protocol Fluidics Station 400 protocol EukGE-WS2v5 (Affymetrix) was performed to wash and stain arrays. The arrays were scanned on GeneChip Scanner 3000 7G (Affymetrix).
Description Rec12 / WCE, meiosis 5h, pht1D cells.
Data processing Data were quantile normalized and smoothed with Affymetrix Tiling Analysis Software.
Processed data files: *.bar
 
Submission date Dec 21, 2018
Last update date Dec 21, 2021
Contact name Shintaro Yamada
E-mail(s) yamadas@mskcc.org
Organization name MEMORIAL SLOAN-KETTERING CANCER CENTER
Department MOLECULAR BIOLOGY PROGRAM
Street address 1275 York Avenue
City New York
State/province New York
ZIP/Postal code 10065
Country USA
 
Platform ID GPL7715
Series (1)
GSE124293 Maintenance of meiotic crossover against reduced DSB formation in fission yeast lacking histone H2A.Z

Supplementary file Size Download File type/resource
GSM3528288_Rec12_pht1D.CEL.gz 12.8 Mb (ftp)(http) CEL
GSM3528288_Rec12_pht1D.bar.gz 5.7 Mb (ftp)(http) BAR
GSM3528288_WCE.CEL.gz 13.1 Mb (ftp)(http) CEL
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap