|
| Status |
Public on Jul 22, 2020 |
| Title |
LATS1/2 KO LEC-3 |
| Sample type |
SRA |
| |
|
| Source name |
Lacteal LECs
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
developmental stage: Adult
genotype: PDGFRb+-CreERT2-Tomato+-Lats1f/f Lats2 f/f
cell type: Lacteal LECs
sorting method: FACS sorted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 whole genome using Bowtie2
Read count extraction and nomalization were performed using edgeR.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Dec 28, 2018 |
| Last update date |
Jul 22, 2020 |
| Contact name |
Hyunsoo Cho |
| E-mail(s) |
hyunsooj325@kaist.ac.kr
|
| Organization name |
IBS/KAIST
|
| Street address |
Yuseonggu
|
| City |
Daejeon |
| ZIP/Postal code |
Daejeon |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE124471 |
Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 [RNA-seq] |
| GSE124488 |
Bulk RNA-sequencing and Single-cell RNA-sequencing of adult mice intestinal stromal cells and lacteal LECs with PDGFRb-specific deletion of LATS1/2 |
|
| Relations |
| BioSample |
SAMN10660654 |
| SRA |
SRX5187723 |
