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Sample GSM355176 Query DataSets for GSM355176
Status Public on Jun 01, 2011
Title Training2
Sample type RNA
 
Source name Pretreatment sample_Training set
Organism Homo sapiens
Characteristics Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
Description Training set to identify the signature for the intrinsic resistance to CF
This sample uses the same CEL data as GSM355321
Data processing The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
 
Submission date Dec 24, 2008
Last update date Jun 01, 2011
Contact name Jeffrey E Green
E-mail(s) JEGreen@mail.nih.gov
Phone 3014355193
Fax 3014968709
Organization name NCI
Street address Rm 4054, 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL571
Series (2)
GSE14208 Clinical response of metastatic gastric cancer patients to cisplatin and fluorouracil (CF) combination chemotherapy
GSE14210 Expression data from human endoscopic biopsy samples
Relations
Alternative to GSM355321

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 9.751724962
1053_at 6.608521837
117_at 7.375311445
121_at 7.771026254
1255_g_at 4.238925877
1294_at 7.187737002
1316_at 5.072774503
1320_at 5.458789318
1405_i_at 8.085039435
1431_at 4.529999324
1438_at 5.72100654
1487_at 7.650557983
1494_f_at 5.701016489
1598_g_at 9.222990246
160020_at 8.950920569
1729_at 7.921147967
177_at 6.302415746
1773_at 5.112325333
179_at 8.038326795
1861_at 6.643887721

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM355176.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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