|
Status |
Public on Sep 30, 2019 |
Title |
Aptamer-3 |
Sample type |
SRA |
|
|
Source name |
Aptamer library
|
Organism |
Homo sapiens |
Characteristics |
sample type: synthetic construct molecule subtype: ssDNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ssDNA aptamers specific towards amyloid-β was selected by SELEX strategy from random aptamer library. The amyloid-β specific ssDNA selected was cloned into pGMET vector by TA cloning strategy and sequenced.
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
AB 5500xl Genetic Analyzer |
|
|
Description |
The aptamer-3 processed from the forward and reverse scanned raw file
|
Data processing |
ssDNA sequence retrieval from sequence scanner software v2.0 ssDNA linear chain generation using Discover studio 2016 version MD simulation of the linear ssDNA using Gromacs 2018 version and generation of 3D structure MD simulation of aptamer against amyloid-β peptide Supplementary_files_format_and_content: PDB files contains the three dimensional structure of the aptamer
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|
|
Submission date |
Jan 09, 2019 |
Last update date |
Oct 01, 2019 |
Contact name |
Nikhil Maroli |
E-mail(s) |
scinikhil@gmail.com
|
Phone |
7200515138
|
Organization name |
DRDO BU CLS
|
Department |
Physics
|
Lab |
Computational Biology
|
Street address |
Bharathiar University
|
City |
Coimbatore |
State/province |
TAMIL NADU |
ZIP/Postal code |
670691 |
Country |
India |
|
|
Platform ID |
GPL16288 |
Series (1) |
GSE124865 |
ssDNA aptamers against amyloid-β peptide as biomarker and inhibtor [SELEX] |
|
Relations |
BioSample |
SAMN10719282 |