|
| Status |
Public on Jan 25, 2019 |
| Title |
Spl Treg rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
Treg
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell type: Treg
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Tissues were surgically removed and mashed through 70micron strainers, cells were sorted on an Aria cell sorter and RNA was extracted using RLT buffer
RNA libraries were constructed using previously published protocols using Illumina adaptors (Sage Nature Immunology 2016)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Basecalling was performed using Illumina Software
Reads were aligned to the mouse genome mm10 using CLC Genomics software suite
Reads per million were generated for each sample
Genome_build: mm10
Supplementary_files_format_and_content: Tab-deliminated file
|
| |
|
| Submission date |
Jan 09, 2019 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Peter Sage |
| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine/Renal
|
| Lab |
Sage Lab
|
| Street address |
221 Longwood Ave
|
| City |
Boston |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE124883 |
FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program [tissue] |
| GSE124884 |
FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program |
|
| Relations |
| BioSample |
SAMN10719837 |
| SRA |
SRX5236202 |
