NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3557908 Query DataSets for GSM3557908
Status Public on Jan 25, 2019
Title Spl Tfr rep 1
Sample type SRA
 
Source name Tfr
Organism Mus musculus
Characteristics tissue: Spleen
cell type: Tfr
Extracted molecule polyA RNA
Extraction protocol Tissues were surgically removed and mashed through 70micron strainers, cells were sorted on an Aria cell sorter and RNA was extracted using RLT buffer
RNA libraries were constructed using previously published protocols using Illumina adaptors (Sage Nature Immunology 2016)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Basecalling was performed using Illumina Software
Reads were aligned to the mouse genome mm10 using CLC Genomics software suite
Reads per million were generated for each sample
Genome_build: mm10
Supplementary_files_format_and_content: Tab-deliminated file
 
Submission date Jan 09, 2019
Last update date Jan 25, 2019
Contact name Peter Sage
Organization name Brigham and Women's Hospital
Department Medicine/Renal
Lab Sage Lab
Street address 221 Longwood Ave
City Boston
ZIP/Postal code 02115
Country USA
 
Platform ID GPL19057
Series (2)
GSE124883 FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program [tissue]
GSE124884 FoxP3 and Ezh2 Regulate Tfr cell Suppressive Function and Transcriptional Program
Relations
BioSample SAMN10719878
SRA SRX5236203

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap