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Sample GSM357409 Query DataSets for GSM357409
Status Public on Jun 02, 2009
Title early cycle 15 haploid embryos, biological rep2
Sample type RNA
 
Source name haploid embryos, 15min after the nuclear division of cycle 14
Organism Drosophila melanogaster
Characteristics Age: early cycle 15
Genotype: maternal haploid;histone H2-GFP parents
Treatment protocol Embryos were identified under Epi fluorescent microscope, timed from telophase of the previous cycle to 15min and 40min later respectively, hand-selected and frozen by dry-ice cold heptane. Triplicates of about 50 embryos each with right age were collected for each time point.
Growth protocol Both diploid and haploid flies were raised at 25C to lay embryos on apple juice agar plate for 2-3hours.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
Scan protocol Gene chips were scaned using Affymetrix GeneChip Scanner 3000.
Description Expression data from precisely staged blastula haploid Drosophila embryos.
Biological replicate 2 of 3.
Data processing The data were analyzed with Microarray Suite using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 05, 2009
Last update date Aug 28, 2018
Contact name Xuemin Lu
E-mail(s) xueminlu@princeton.edu
Organization name Princeton University
Department Molecular biology
Lab Wieschaus
Street address Washington Rd
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
 
Platform ID GPL1322
Series (1)
GSE14287 Expression data from precisely staged blastula wild-type and haploid Drosophila embryos
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE MAS5-calculated signal intensity
ABS_CALL Call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 181.6 P
AFFX-BioB-M_at 288.6 P
AFFX-BioB-3_at 176.6 P
AFFX-BioC-5_at 527.5 P
AFFX-BioC-3_at 407.9 P
AFFX-BioDn-5_at 1343.5 P
AFFX-BioDn-3_at 2681 P
AFFX-CreX-5_at 7805.5 P
AFFX-CreX-3_at 8669.6 P
AFFX-DapX-5_at 204.9 P
AFFX-DapX-M_at 357.6 P
AFFX-DapX-3_at 461.3 P
AFFX-LysX-5_at 27.3 P
AFFX-LysX-M_at 38.2 A
AFFX-LysX-3_at 102.7 P
AFFX-PheX-5_at 39.9 P
AFFX-PheX-M_at 52.3 P
AFFX-PheX-3_at 71.1 P
AFFX-ThrX-5_at 44.2 P
AFFX-ThrX-M_at 79.4 P

Total number of rows: 18952

Table truncated, full table size 336 Kbytes.




Supplementary file Size Download File type/resource
GSM357409.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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