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Sample GSM357418 Query DataSets for GSM357418
Status Public on Jun 02, 2009
Title early cycle 14 wild-type embryos, biological rep2
Sample type RNA
 
Source name wild-type embryos, 15min after the nuclear division of cycle 13
Organism Drosophila melanogaster
Characteristics Age: early cycle 14
Genotype: w;histone H2-GFP parents
Treatment protocol Embryos were identified under Epi fluorescent microscope, timed from telophase of the previous cycle to 15min and 40min later respectively, hand-selected and frozen by dry-ice cold heptane. Triplicates of about 50 embryos each with right age were collected for each time point.
Growth protocol Both diploid and haploid flies were raised at 25C to lay embryos on apple juice agar plate for 2-3hours.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
Scan protocol Gene chips were scaned using Affymetrix GeneChip Scanner 3000.
Description Expression data from precisely staged wild-type (diploid) Drosophila embryos.
Biological replicate 2 of 3.
Data processing The data were analyzed with Microarray Suite using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 05, 2009
Last update date Aug 28, 2018
Contact name Xuemin Lu
E-mail(s) xueminlu@princeton.edu
Organization name Princeton University
Department Molecular biology
Lab Wieschaus
Street address Washington Rd
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
 
Platform ID GPL1322
Series (1)
GSE14287 Expression data from precisely staged blastula wild-type and haploid Drosophila embryos
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE MAS5-calculated signal intensity
ABS_CALL Call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 198.7 P
AFFX-BioB-M_at 274.6 P
AFFX-BioB-3_at 196 P
AFFX-BioC-5_at 498.3 P
AFFX-BioC-3_at 409.7 P
AFFX-BioDn-5_at 1459.6 P
AFFX-BioDn-3_at 2904.6 P
AFFX-CreX-5_at 8839.5 P
AFFX-CreX-3_at 10231.4 P
AFFX-DapX-5_at 269.2 P
AFFX-DapX-M_at 505.3 P
AFFX-DapX-3_at 667.3 P
AFFX-LysX-5_at 30.2 P
AFFX-LysX-M_at 51.8 A
AFFX-LysX-3_at 143.1 P
AFFX-PheX-5_at 55.1 P
AFFX-PheX-M_at 82.9 P
AFFX-PheX-3_at 88.4 P
AFFX-ThrX-5_at 51.7 P
AFFX-ThrX-M_at 98.6 P

Total number of rows: 18952

Table truncated, full table size 339 Kbytes.




Supplementary file Size Download File type/resource
GSM357418.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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