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Sample GSM357437 Query DataSets for GSM357437
Status Public on Jan 04, 2010
Title WT_5_no_treatment
Sample type RNA
 
Source name WT_no_serum_treatment
Organism Mus musculus
Characteristics cultured myocytes
Biomaterial provider The University of Washington (Seattle, WA).
Treatment protocol Transgenic mice overexpressing CHF1/Hey2 in the myocardium under the control of the mlc2v promoter and neonatal mouse cardiac myocyte culture was performed as previously described. To induce hypertrophy, neonatal myocytes in serum free medium were exposed to media with 20% fetal bovine serum for 24 hours.
Extracted molecule total RNA
Extraction protocol RNA was isolated from cultured myocytes by extraction with a commercially available kit (Qiagen) according to the manufacturer’s instructions.
Label biotin
Label protocol For microarray hybridization, RNA was processed for hybridization to Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays through a core facility in the Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer’s instructions.
 
Hybridization protocol The standard hybridization protocol was used as recommended by Affymterix.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
Description cultured myocytes.
Data processing Affymetrix Expression Console v1.1 RMA normalization log2
 
Submission date Jan 05, 2009
Last update date Sep 05, 2017
Contact name James William MacDonald
E-mail(s) jmacdon@uw.edu
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
 
Platform ID GPL6246
Series (1)
GSE14288 Transcription Factor CHF1/Hey2 Regulates Specific Gene Sets in Serum Stimulated Primary Cardiac Myocytes

Data table header descriptions
ID_REF
VALUE RMA normalized signal log2

Data table
ID_REF VALUE
10344614 5.177277
10344616 2.966272
10344620 3.307753
10344622 6.017952
10344624 8.776157
10344633 9.518723
10344637 8.290097
10344653 3.273165
10344658 6.877631
10344674 3.431976
10344679 4.790191
10344707 7.409419
10344713 9.36509
10344715 3.788206
10344717 4.161845
10344719 5.712506
10344721 3.013799
10344723 5.747167
10344725 4.64832
10344741 9.70247

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM357437.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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