|
| Status |
Public on Dec 01, 2010 |
| Title |
wt control |
| Sample type |
RNA |
| |
|
| Source name |
wt control
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
wt arabidopsis leaves
|
| Biomaterial provider |
our lab
|
| Treatment protocol |
control
|
| Growth protocol |
Plants were grown under white light in a 10-h-light/14-h-dark cycle (short day ) at 21°C.
6 weeks old plants
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using RNeasy plant Mini Kit (Qiagen) according to the manufacture's protocol.
|
| Label |
Biotin
|
| Label protocol |
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix), in the Weizmann Institute microarray unit
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip ATH1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GC3000 7G.
|
| Description |
wt control
|
| Data processing |
RMA via the PARTEK software
|
| |
|
| Submission date |
Jan 08, 2009 |
| Last update date |
Dec 09, 2009 |
| Contact name |
Robert Fluhr |
| E-mail(s) |
robert.fluhr@weizmann.ac.il
|
| Phone |
972-8-9342175
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Plant Sciences
|
| Street address |
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE14332 |
wt and opr3 control and systemic wounding |
|
